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Boldness in adult zebrafish : place preference protocol.

Boldness in adult zebrafish : place preference protocol.

Can also be used to assess anxiety although our test set-up still needs to be validated with anxiolytic drugs. More information on ZF-HEALTH.

Materials

  • Place-preference tank
  • Stopwatch to record time
  • Tracking system (Viewpoint)
  • Adult zebrafish
Place Preference tank

Conditions

  • Start of experiments : Afternoon
  • Duration:10mn per fish
  • Habituation : not necessary
  • Temperature of water : 28°C
  • Temperature of room : 22°C
  • Lighting : Infrared light below tank

Parameters

  • Age of fish : from 3 months onwards
  • Strain : all
  • Size of group : 15 fish per genotype
  • Drug exposure : possible, for various times and concentrations
  • Drug administration by injection : possible
  • Ability to film to the top or side

Measure

We can measure the time spent in different areas of the tank (defined by the user), and the swimming speed in each of these areas during the experiment.

Cost

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