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Boldness in adult zebrafish : predator avoidance

Boldness in adult zebrafish : predator avoidance

Can also be used to assess anxiety, although our test set-up still needs to be validated with anxiolytic drugs. Choice between the place preference and predator avoidance protocols can be made by the users. More information on ZF-HEALTH.

Materials

  • Tank in plexiglass
  • Stopwatch to record time
  • Fake predator
  • Tracking system (Viewpoint)
  • Adult zebrafish

Conditions

  • Start of experiments : Afternoon
  • Duration:10mn per fish
  • Habituation : not necessary
  • Temperature of water : 28°C
  • Temperature of room : 22°C
  • Lighting : Infrared light below Tank

Parameters

  • Age of fish : from 3 months onwards
  • Strain : all
  • Size of group : 15 fish per genotype
  • Drug exposure : possible, for various times and concentrations
  • Drug administration by injection : possible
  • Ability to film to the top or side

Measure

We can measure the time spent in different areas of the tank (defined by the user), notably in the predator area, and the swimming speed in each area during the experiment.

Cost

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