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AMAGEN

UMS AMAGEN (UMS 3504 CNRS / UMS 1374 INRA) is located on 2 sites :
Gif-sur-Yvette and Jouy-en-Josas. Its aim is to promote the utilization of aquatic animal models. AMAGEN has developed two models of teleost fishes:medaka (Oryzias latipes) and zebrafish (Danio rerio). The issues of modern biology involve the implementation of techniques allowing comparative analysis of living organisms.

Among vertebrates, the fish model displays easy high density breeding (short generation time), favorable biological peculiarities (accessibility and transparency of the eggs, genome sequenced for both species used on the platform), original technological approaches (confocal and two-photon microscopy), as well as a wide accessibility to fully transparent larvae usable up to 5 days after fertilization.

Transgenesis is an essential technique for studies in vivo. In a research and development process improving the transgenesis techniques in both species, AMAGEN will provide customized transgenic lines to European research teams.

Since its creation in 2009, AMAGEN platform has conducted research projects in the area of high specificity nucleases to develop targeted transgenesis and genome editing methods. In the frame of TEFOR, an infrastructure laureate of Future Investments in 2012 and focusing on non-mammalian animal models, AMAGEN has increased its capacity to produce zebrafish lines. The platform now proposes techniques to generate zebrafish lines via genome editing and KO (Knock-Out) models.

Some recent publications :

RESAMA: A Network for Monitoring Health and Husbandry Practices in Aquatic Research Facilities. Legendre L, Guillet B, Leguay E, Meunier E, Labrut S, Keck N, Bardotti M, Michelet L, Sohm F.
Zebrafish. 2016 May 18
PMID: 27192449

 

Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases. Renaud JB, Boix C, Charpentier M, De Cian A, Cochennec J, Duvernois-Berthet E, Perrouault L, Tesson L, Edouard J, Thinard R, Cherifi Y, Menoret S, Fontanière S, de Crozé N, Fraichard A, Sohm F, Anegon I, Concordet JP, Giovannangeli C.
Cell Rep. 2016 Mar 8;14(9):2263-72
PMID: 26923600

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A TALEN-Exon Skipping Design for a Bethlem Myopathy Model in Zebrafish.
Radev Z, Hermel JM, Elipot Y, Bretaud S, Arnould S, Duchateau P, Ruggiero F, Joly JS, Sohm F.
PLoS One. 2015 Jul 29;10(7):e0133986
PMID: 26221953

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