Le cerveau multidimensionnel

Accueil > Departments > Development and Evolution of the Brain > Ronald Melki Team




- Walsh RR, Krismer F, Galpern WR, Wenning GK, Low PA, Halliday G, Koroshetz WJ, Holton J, Quinn NP, Rascol O, Shaw LM, Eidelberg D, Bower P, Cummings JL, Abler V, Biedenharn J, Bitan G, Brooks DJ, Brundin P, Fernandez H, Fortier P, Freeman R, Gasser T, Hewitt A, Höglinger GU, Huentelman MJ, Jensen PH, Jeromin A, Kang UJ, Kaufmann H, Kellerman L, Khurana V, Klockgether T, Kim WS, Langer C, LeWitt P, Masliah E, Meissner W, Melki R, Ostrowitzki S, Piantadosi S, Poewe W, Robertson D, Roemer C, Schenk D, Schlossmacher M, Schmahmann JD, Seppi K, Shih L, Siderowf A, Stebbins GT, Stefanova N, Tsuji S, Sutton S, Zhang J. Recommendations of the Global Multiple System Atrophy Research Roadmap Meeting. Neurology. 2017 Dec 13. pii : 10.1212/WNL.0000000000004798. doi : 10.1212/WNL.0000000000004798. [Epub ahead of print] Review.

- Brundin P., and Melki R. Prying into the Prion Hypothesis for Parkinson’s Disease. J Neurosci. 2017 Oct 11 ;37(41):9808-9818. doi : 10.1523/JNEUROSCI.1788-16.2017.

- Wang K., Melki R., and Kabani M. A prolonged chronological lifespan is an unexpected benefit of the [PSI+] prion in yeast. PLoS One. 2017 Sep 14 ;12(9):e0184905. doi : 10.1371/journal.pone.0184905. eCollection 2017

- Loria F., Vargas J.Y., Bousset L., Syan S., Salles A., Melki R., and Zurzolo C. α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading. Acta Neuropathol. 2017 Jul 19. doi : 10.1007/s00401-017-1746-2. [Epub ahead of print]

- Shrivastava A.N., Aperia A., Melki R., and Triller A. Physico-Pathologic Mechanisms Involved in Neurodegeneration : Misfolded Protein-Plasma Membrane Interactions. Neuron. 2017 Jul 5 ;95(1):33-50. doi : 10.1016/j.neuron.2017.05.026.

- Flavin W.P., Bousset L., Green Z.C., Chu Y., Skarpathiotis S., Chaney M.J., Kordower J.H., Melki R., and Campbell E.M. Endocytic vesicle rupture is a conserved mechanism of cellular invasion by amyloid proteins. Acta Neuropathol. 2017 May 19. doi : 10.1007/s00401-017-1722-x. [Epub ahead of print]

- Melki R. How the shapes of seeds can influence pathology. Neurobiol Dis. 2017 Mar 29. pii : S0969-9961(17)30060-8. doi : 10.1016/j.nbd.2017.03.011. [Epub ahead of print]


- Makky A., Bousset L., Polesel-Maris J., and Melki R. Nanomechanical properties of distinct fibrillar polymorphs of the protein α-synuclein. Sci Rep. 2016 Nov 30 ;6:37970. doi : 10.1038/srep37970 (recommended by F1000)

- Pampalakis G., Sykioti V.S., Ximerakis M., Stefanakou-Kalakou I., Melki R., Vekrellis K., and Sotiropoulou G. KLK6 proteolysis is implicated in the turnover and uptake of extracellular alpha-synuclein species. Oncotarget. 2016 Nov 10. doi : 10.18632/oncotarget.13264. [Epub ahead of print]

- Tardivel M., Bégard S., Bousset L., Dujardin S., Coens A., Melki R., Buée L., and Colin M. Tunneling nanotube (TNT)-mediated neuron-to neuron transfer of pathological Tau protein assemblies. Acta Neuropathol Commun. 2016 Nov 4 ;4(1):117

- Abounit S., Bousset L., Loria F., Zhu S., de Chaumont F., Pieri L., Olivo-Marin J.C., Melki R., and Zurzolo C. Tunneling nanotubes spread fibrillar α-synuclein by intercellular trafficking of lysosomes. EMBO J. 2016 Aug 22. pii : e201593411. [Epub ahead of print]

- Eychenne T., Novikova E., Barrault M-B., Alibert O., Boschiero C., Peixeiro N., Cornu D., Redeker V., Kuras L., Nicolas P., Werner M., and Soutourina J. Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture. Genes Dev. 2016 Sep 15 ;30(18):2119-2132. Epub 2016 Sep 29.

- Rémion A., Khoder-Agha F., Cornu D., Argentini M., Redeker V., and Mirande M. Identification of protein interfaces within the multi-aminoacyl-tRNA synthetase complex : the case of lysyl-tRNA synthetase and the scaffold protein p38. FEBS Open Bio. 2016 May 25 ;6(7):696-706. doi : 10.1002/2211-5463.12074. eCollection 2016.

- Kakkar V., Månsson C., de Mattos E.P., Bergink S., van der Zwaag M., van Waarde M.A., Kloosterhuis N.J., Melki R., van Cruchten R.T., Al-Karadaghi S., Arosio P., Dobson C.M., Knowles T.P., Bates G.P., van Deursen J.M., Linse S., van de Sluis B., Emanuelsson C., and Kampinga H.H. The S/T-Rich Motif in the DNAJB6 Chaperone Delays Polyglutamine Aggregation and the Onset of Disease in a Mouse Model. Mol Cell. 2016 Apr 12. pii : S1097-2765(16)00227-6. doi : 10.1016/j.molcel.2016.03.017. [Epub ahead of print]

- Pieri L., Madiona K., and Melki R. Structural and functional properties of prefibrillar α-synuclein oligomers. Sci Rep. 2016 Apr 14 ;6:24526. doi : 10.1038/srep24526

- Kabani M., and R. Melki. More than just trash bins ? Potential roles for extracellular vesicles in the vertical and horizontal transmission of yeast prions. Current Genetics 2016 May ;62(2):265-70. doi : 10.1007/s00294-015-0534-6. Epub 2015 Nov 9

- Yi Z., Manil-Ségalen M., Sago L., Glatigny A., Redeker V., Legouis R., and Mucchielli-Giorgi M.H. SAFER, an analysis method of quantitative proteomic data, reveals new interactors of the C. elegans autophagic protein LGG-1. J Proteome Res. 2016 Mar 21. [Epub ahead of print]

- Shrivastava A.N., Redeker V., Fritz N., Pieri L., Almeida L.G., Spolidoro M., Liebmann T., Bousset L., Renner M., Léna C., Aperia A., Melki R., and Triller A. Data in support of the identification of neuronal and astrocyte proteins interacting with extracellularly applied oligomeric and fibrillar α-synuclein assemblies by mass spectrometry. Data Brief. 2016 Feb 12 ;7:221-8

- Hai J., Serradji N., Mouton L., Redeker V., Cornu D., El Hage Chahine J.M., Verbeke P., and Hémadi M. Targeted Delivery of Amoxicillin to C. trachomatis by the Transferrin Iron Acquisition Pathway. PLoS One. 2016 Feb 26 ;11(2):e0150031

- Brahic M., Bousset L., Bieri G., Melki R., and Gitler A.D. Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1. Acta Neuropathol. 2016 Jan 28. [Epub ahead of print]

- Aillaud C., Bosc C., Saoudi Y., Denarier E., Peris L., Sago L., Taulet N., Cieren A., Tort O., Magiera M.M., Janke C., Redeker V., Andrieux A., and Moutin M.J. Evidence for new C-terminally truncated variants of α- and β-tubulins. Mol Biol Cell. 2016 Jan 6. pii : mbc.E15-03-0137. [Epub ahead of print]

- Monsellier E., Bousset L., and Melki R. α-Synuclein and huntingtin exon 1 amyloid fibrils bind laterally to the cellular membrane. Sci Rep. 2016 Jan 13 ;6:19180. doi : 10.1038/srep19180

- Pieri L., Chafey P., Le Gall M., Clary G., Melki R., and V. Redeker. Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies. Biochim Biophys Acta. 2016 Jan ;1860(1 Pt A):8-19. doi : 10.1016/j.bbagen.2015.10.007. Epub 2015 Oct 22.


- Bousset L., Brundin P., Böckmann A., Meier B., and Melki R. An efficient procedure for removal and inactivation of alpha-synuclein assemblies from laboratory materials. J Parkinsons Dis. 2015 Nov 30. [Epub ahead of print] (recommended by F1000)

- Shrivastava A.M., Redeker V., Fritz N., Pieri L., Almeida L.G., Spolidoro M., Liebmann T., Bousset L., Renner M., Léna C., Aperia A., Melki R., and A. Triller. α-synuclein assemblies sequester neuronal α3-Na+/K+-ATPase and impair Na+ gradient. EMBO J. 2015 Aug 31. pii : e201591397. [Epub ahead of print] (FRONT COVER IMAGE)

- Verasdonck J., Bousset L., Gath J., Melki R., Böckmann A., and B.H. Meier. Further exploration of the conformational space of α-synuclein fibrils : solid-state NMR assignment of a high-pH polymorph. Biomol NMR Assign. 2015 Aug 30. [Epub ahead of print]

- Kabani M., and R. Melki. Sup35p in Its Soluble and Prion States Is Packaged inside Extracellular Vesicles. MBio. 2015 Aug 18 ;6(4). pii : e01017-15. doi : 10.1128/mBio.01017-15.

- Eyboulet F., Wydau-Dematteis S., Eychenne T., Alibert O., Neil H., Boschiero C., Nevers M-C., Volland H., Cornu D., Redeker V., Werner M., and J. Soutourina. Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo. Nucleic Acids Res. 2015 Aug 3. pii : gkv782. [Epub ahead of print]

- Wang K., Redeker V., Madiona K., Melki R., and M. Kabani. The 26S Proteasome Degrades the Soluble but Not the Fibrillar Form of the Yeast Prion Ure2p In Vitro. PLoS One. 2015 Jun 26 ;10(6):e0131789. doi : 10.1371/journal.pone.0131789

- Ruiz-Arlandis G., Pieri L., Bousset L., and R. Melki. Binding, internalization and fate of Huntingtin Exon1 fibrillar assemblies in mitotic and non-mitotic neuroblastoma cells. Neuropathol Appl Neurobiol. 2015 Jun 25. doi : 10.1111/nan.12258. [Epub ahead of print]

- Peelaerts W., Bousset L., Van der Perren A., Moskalyuk A., Pulizzi R., Giugliano M., Van den Haute C., Melki R., and V. Baekelandt. α-Synuclein strains cause distinct synucleinopathies after local and systemic administration. Nature. 2015 Jun 18 ;522(7556):340-4. doi : 10.1038/nature14547. Epub 2015 Jun 10 (recommended by F1000)

- Garten M., Prévost C., Cadart C., Gautier R., Bousset L., Melki R., Bassereau P., and S. Vanni. Methyl-branched lipids promote the membrane adsorption of α-synuclein by enhancing shallow lipid-packing defects. hys Chem Chem Phys. 2015 Jun 28 ;17(24):15589-97. doi : 10.1039/c5cp00244c. Epub 2015 Mar 31

- R. Melki. Role of Different Alpha-Synuclein Strains in Synucleinopathies, Similarities with other Neurodegenerative Diseases. J Parkinsons Dis. 2015 Mar 10. [Epub ahead of print]

- Nury C., Redeker V., Dautrey S., Romieu A., van der Rest G., Renard P-Y., Melki R., and J. Chamot-Rooke. A novel bio-orthogonal cross-linker for improved protein/protein interaction analysis. Anal Chem. 2015 Feb 3 ;87(3):1853-60. doi : 10.1021/ac503892c. Epub 2015 Jan 12

- Monsellier E., Redeker V., Ruiz-Arlandis G., Bousset L., and R. Melki. Molecular interaction between the chaperone Hsc70 and the N-terminal flank of huntingtin exon 1 modulates aggregation. J Biol Chem. 2015 Jan 30 ;290(5):2560-76. doi : 10.1074/jbc.M114.603332. Epub 2014 Dec 10


- Boza-Serrano A., Reyes J.F., Rey N.L., Leffler H., Bousset L., Nilsson U., Brundin P., Venero J., Burguillos M.,and T. Deierborg. The role of Galectin-3 in α-synuclein-induced microglial activation. Acta Neuropathol Commun. 2014 Nov 12 ;2(1):156. [Epub ahead of print]

- Bousset L., Mary C., Brooks M.A., Scherrer A., Strub K., and S. Cusack. Crystal structure of a signal recognition particle Alu domain in the elongation arrest conformation. RNA. 2014 Dec ;20(12):1955-62

- Holmqvist S., Chutna O., Bousset L., Aldrin-Kirk P., Li W., Björklund T., Wang Z.Y., Roybon L., Melki R., and J.Y. Li. Direct evidence of Parkinson pathology spread from the gastrointestinal tract to the brain in rats. Acta Neuropathol. 2014 Dec ;128(6):805-20

- Dujardin S., Bégard S., Caillierez R., Lachaud C., Delattre L., Carrier S., Loyens A., Galas M.C., Bousset L., Melki R., Aurégan G., Hantraye P., Brouillet E., Buée L.,and M.Colin. Ectosomes : a new mechanism for non-exosomal secretion of tau protein. PLoS One. 2014 Jun 27 ;9(6):e100760. doi : 10.1371/journal.pone.0100760. eCollection 2014

- Bousset L., Brewee C., Melki R., and F. Migliardo. Dynamical properties of α-synuclein in soluble and fibrillar forms by Quasi Elastic Neutron Scattering. Biochim Biophys Acta. 2014 Apr 21. pii : S1570-9639(14)00096-X. doi : 10.1016/j.bbapap.2014.04.010. [Epub ahead of print]

- Renner M., and R. Melki. Protein aggregation and prionopathies. Pathol Biol (Paris). 2014 Mar 31. pii : S0369-8114(14)00033-9. doi : 10.1016/j.patbio.2014.01.003. [Epub ahead of print]

- Bétemps D., Verchère J., Brot S., Morignat E., Bousset L., Gaillard D., Lakhdar L., Melki R., and T. Baron. Alpha-synuclein spreading in M83 mice brain revealed by detection of pathological alpha-synuclein by enhanced ELISA. Acta Neuropathol Commun. 2014 Mar 13 ;2(1):29. [Epub ahead of print]

- Diepenbroek M., Casadei N., Esmer H., Saido T.C., Takano J., Kahle P.J., Nixon R.A., Rao M.V., Melki R., Pieri L., Helling S., Marcus K., Krueger R., Masliah E., Riess O., and S. Nuber. Overexpression of the Calpain-specific Inhibitor Calpastatin reduces human alpha-Synuclein Processing, Aggregation and Synaptic Impairment in [A30P]αSyn transgenic mice. Hum Mol Genet. 2014 Mar 11. [Epub ahead of print]

- Gath J., Bousset L., Habenstein B., Melki R., Böckmann A., and B.H. Meier. Unlike Twins : An NMR Comparison of Two α-Synuclein Polymorphs Featuring Different Toxicity. PLoS One. 2014 Mar 5 ;9(3):e90659. doi : 10.1371/journal.pone.0090659. eCollection 2014

- Kabani M., Redeker V., and R. Melki. A role for the proteasome in the turnover of Sup35p and in [PSI+] prion propagation. Mol Microbiol. 2014 May ;92(3):507-28. doi : 10.1111/mmi.12572. Epub 2014 Mar 17. (FRONT COVER IMAGE)

- Reyes J.F., Rey N.L., Bousset L., Melki R., Brundin P., and E. Angot.Alpha-synuclein transfers from neurons to oligodendrocytes. Glia. 2014 Mar ;62(3):387-98. doi : 10.1002/glia.22611. Epub 2013 Dec 31.

- Månsson C., Kakkar V., Monsellier E., Sourigues Y., Härmark J., Kampinga H.H., Melki R., and C. Emanuelsson. DNAJB6 is a peptide-binding chaperone which can suppress amyloid fibrillation of polyglutamine peptides at substoichiometric molar ratios. Cell Stress Chaperones.2014 Mar ;19(2):227-39. doi:10.1007/s12192-013-0448-5. Epub 2013 Aug 1.


- Luckgei N., Schütz A.K., Bousset L., Habenstein B., Sourigues Y., Gardiennet C., Meier B.H., Melki R., and A. Böckmann. The Conformation of the Prion Domain of Sup35 p in Isolation and in the Full-Length Protein. Angew Chem Int Ed Engl. 2013 Nov 25 ;52(48):12741-4. doi : 10.1002/anie.201304699. Epub 2013 Oct 9

- Gath J., Bousset L., Habenstein B., Melki R., Meier B.H., and A. Böckmann. Yet another polymorph of α-synuclein : solid-state sequential assignments. Biomol NMR Assign. 2013 Oct 10. [Epub ahead of print]

- Bousset L., Pieri L., Ruiz-Arlandis G., Gath J., Jensen P.H., Habenstein B., Madiona K., Olieric V., Böckmann A., Meier B.H., and R. Melki. Structural and functional characterization of two alpha-synuclein strains. Nature Communications 2013 Oct 10 ;4:2575. doi : 10.1038/ncomms3575.

- Pucciarelli S., Chiappori F., Sparvoli D., Milanesi L., Miceli C., and R. Melki. Tubulin folding : the special case of a beta-tubulin isotype from the Antarctic psychrophilic ciliate Euplotes focardii. Polar Biology 21 Aug 2013 (DOI 10.1007/s00300-013-1390-9)

- Schütz A.K., Habenstein B., Luckgei N., Bousset L., Sourigues Y., Nielsen A.B., Melki R., Böckmann A., and B.H. Meier. Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p. Biomol NMR Assign. 2013 Aug 14. [Epub ahead of print]

- Luckgei N., Schütz A.K., Habenstein B., Bousset L., Sourigues Y., Melki R., Meier B.H., and A. Böckmann. Solid-state NMR sequential assignments of the amyloid core of Sup35pNM. Biomol NMR Assign. 2013 Aug 10. [Epub ahead of print]

- Rey N.L., Petit G.H., Bousset L., Melki R., and P. Brundin. Transfer of human α-synuclein from the olfactory bulb to interconnected brain regions in mice. Acta Neuropathol. 2013 Oct ;126(4):555-73. doi:10.1007/s00401-013-1160-3. Epub 2013 Aug 8

- Shrivastava A.N., Kowalewski J.M., Renner M., Bousset L., Koulakoff A., Melki R., Giaume C., and A. Triller. β-amyloid and ATP-induced diffusional trapping of astrocyte and neuronal metabotropic glutamate type-5 receptors. Glia. Oct ;61(10):1673-86. doi : 10.1002/glia.22548. Epub 2013 Aug 6

- Jakobsson M.E., Moen A., Bousset L., Egge-Jacobsen W., Kernstock S., Melki R.,and P.O. Falnes. Identification and characterization of a novel human methyltransferase modulating Hsp70 function through lysine methylation. J Biol Chem. 2013 Sep 27 ;288(39):27752-63. doi : 10.1074/jbc.M113.483248. Epub 2013 Aug 6

- Bousset L., and Melki R. [Infectious properties of protein aggregates involved in neurodegenerative diseases] [article in french]. Biol Aujourdhui 2013 ;207(1):55-9. doi : 10.1051/jbio/2013004. Epub 2013 May 23

- Boudiaf-Benmammar C., Cresteil T., and R. Melki. The cytosolic chaperonin CCT/TRiC and cancer cell proliferation. PLoS One. 2013 Apr 16 ;8(4):e60895

- Nussbaum-Krammer C.I., Park K.W., Li L., Melki R., Morimoto R.I. Spreading of a prion domain from cell-to-cell by vesicular transport in Caenorhabditis elegans. PLoS Genet. 2013 Mar ;9(3):e1003351. doi : 10.1371/journal.pgen.1003351. Epub 2013 Mar 28.

- Lesage S., Anheim M., Letournel F., Bousset L., Honoré A., Rozas N., Pieri L., Madiona K., Dürr A., Melki R., Verny C., Brice A. ; for the French Parkinson’s Disease Genetics (PDG) Study Group. G51D α-synuclein mutation causes a novel parkinsonian-pyramidal syndrome. Ann Neurol. 2013 Mar 22. doi : 10.1002/ana.23894. [Epub ahead of print]

- Streets A.M., Sourigues Y., Kopito R.R., Melki R., and S.R. Quake. Simultaneous measurement of amyloid fibril formation by dynamic light scattering and fluorescence reveals complex aggregation kinetics. PLoS One. 2013 ;8(1):e54541

- Kabani, M. and C.N. Martineau. Multiple Hsp70 isoforms in the eukaryotic cytosol:evidence for both redundant and specialized functions. Advances in Genome Science : Changing Views on Living Organisms vol. 1, 2013, 426-51


- Freundt E.C., Maynard N., Clancy E.K., Roy S., Bousset L., Sourigues Y., Covert M., Melki R., Kirkegaard K., and M. Brahic. Neuron-to-neuron transmission of α-synuclein fibrils through axonal transport. Ann Neurol. 2012 Oct ;72(4):517-24. doi : 10.1002/ana.23747 (recommended by F1000)

- van der Goot A.T., Zhu W., Vázquez-Manrique R.P., Seinstra R.I., Dettmer K., Michels H., Farina F., Krijnen J., Melki R., Buijsman R.C., Ruiz Silva M., Thijssen K.L., Kema I.P., Neri C., Oefner P.J., and E.A. Nollen. Delaying aging and the aging-associated decline in protein homeostasis by inhibition of tryptophan degradation. Proc Natl Acad Sci U S A. 2012 Aug 27. [Epub ahead of print]

- Richardson K., Denny R., Hughes C., Skilling J., Sikora J., Dadlez M., Manteca A., Jung H.R., Jensen O.N., Redeker V., Melki R., Langridge J.I., and J.P. Vissers. A Probabilistic framework for peptide and protein quantification from data-dependent and data-independent LC-MS proteomics experiments. OMICS. 2012 Aug 7. [Epub ahead of print]

- Mouilleron S., Badet-Denisot M.A., Pecqueur L., Madiona K., Assrir N., Badet B., and B. Golinelli-Pimpaneau. Structural basis for morpheein-type allosteric regulation of E. coli glucosamine-6P synthase : equilibrium between inactive hexamer and active dimer. J Biol Chem. 2012 Jul 31. [Epub ahead of print]

- Redeker V., Pemberton S., Bienvenut W., Bousset L., and R. Melki. Identification of protein interfaces between alpha-synuclein, the principal component of Lewy bodies in Parkinson’s disease, and the molecular chaperones human Hsc70 and the yeast Ssa1p. J Biol Chem. 2012 Jul 26. [Epub ahead of print]

- Habenstein B., Bousset L., Sourigues Y., Kabani M., Loquet A., Meier B.H., Melki R., and A. Böckmann. A Native-Like Conformation for the C-Terminal Domain of the Prion Ure2p Within its Fibrillar Form. Angew Chem Int Ed Engl. 2012 Jul 3. doi : 10.1002/anie.201202093. [Epub ahead of print] (SFBBM : article of the month, February 2013)

- Trevino R.S., Lauckner J.E., Sourigues Y., Pearce M.P., Bousset L., Melki R., and R.R. Kopito. Fibrillar structure and charge determine the interaction of polyglutamine protein aggregates with the cell surface. J Biol Chem. 2012 Jun 29. [Epub ahead of print] (recommended by F1000)

- Pieri L., Madiona K., Bousset L., and R. Melki. Fibrillar α-Synuclein and Huntingtin Exon 1 Assemblies Are Toxic to the Cells. Biophysical J. 2012 Jun 20 ;102(12):2894-905 (Epub 2012 Jun 19)

- Albertini A.A., Mérigoux C., Libersou S., Madiona K., Bressanelli S., Roche S., Lepault J., Melki R., Vachette P., and Y. Gaudin. Characterization of monomeric intermediates during VSV glycoprotein structural transition. PLoS Pathogens 8(2):e1002556 (Epub 2012 Feb 23) (recommended by F1000)

- Ramazzotti M., Monsellier E., Kamoun C., Degl’innocenti D., and R. Melki. Polyglutamine repeats are associated to specific sequence biases that are conserved among eukaryotes. PLoS One 2012 7(2):e30824 (Epub 2012 Feb 1)

- Pemberton S., and R. Melki.The interaction of Hsc70 protein with fibrillar α-Synuclein and its therapeutic potential in Parkinson’s disease. Commun Integr Biol 2012 5(1):94-95

- Bucciantini M., Nosi D., Forzan M., Russo E., Calamai M., Pieri L., Formigli L., Quercioli F., Soria S., Pavone F., Savistchenko J., Melki .R, and M. Stefani. Toxic effects of amyloid fibrils on cell membranes : the importance of ganglioside GM1. FASEB J. 2012 26(2):818-831 (Epub 2011 Nov 9)

- Martineau CN, Le-Dall M-T, Melki R, Beckerich J-M, Kabani M. Molecular and functional characterization of the only known hemiascomycete ortholog of the carboxyl terminus of Hsc70-interacting protein CHIP in the yeast Yarrowia lipolytica. Cell Stress Chaperones 2012 17(2):229-241 (Epub 2011 Oct. 29)

- Gath J, Habenstein B, Bousset L, Melki R, Meier BH, Böckmann A. Solid-state NMR sequential assignments of α-synuclein. Biomol NMR Assign. 2012 Apr ;6(1):51-5 (Epub 2011 Jul 9)


- Kabani M, Melki R. Yeast prions assembly and propagation : Contributions of the prion and non-prion moieties and the nature of assemblies. Prion 2011 5(4):277-284

- Redeker V, Hughes C, Savistchenko J, Vissers JP, Melki R. Qualitative and quantitative multiplexed proteomic analysis of complex yeast protein fractions that modulate the assembly of the yeast prion sup35p. PLoS One. 2011 ;6(9):e23659.

- Pemberton S, Madiona K, Pieri L, Kabani M, Bousset L, Melki R. Hsc70 interaction with soluble and fibrillar alpha-Synuclein. J Biol Chem. 2011 Oct 7 ;286(40):34690-9. Epub 2011 Aug 10.

- Fauquant C, Redeker V, Landrieu I, Wieruszeski JM, Verdegem D, Laprévote O, Lippens G, Gigant B, Knossow M. Systematic identification of tubulin-interacting fragments of the microtubule-associated protein Tau leads to a highly efficient promoter of microtubule assembly. J Biol Chem. Sep 23 ;286(38):33358-68.

- Habenstein B, Wasmer C, Bousset L, Sourigues Y, Schütz A, Loquet A, Meier BH, Melki R, Böckmann A. Extensive de novo solid-state NMR assignments of the 33 kDa C-terminal domain of the Ure2 prion. J Biomol NMR. 2011 Jul 31. [Epub ahead of print]

- Kabani M, Cosnier B, Bousset L, Rousset JP, Melki R, Fabret C. A mutation within the C-terminal domain of Sup35p that affects [PSI+] prion propagation. Mol Microbiol. 2011 Aug ;81(3):640-58. doi : 10.1111/j.1365-2958.2011.07719.x. Epub 2011 Jun 16.

- Hansen C, Angot E, Bergström AL, Steiner JA, Pieri L, Paul G, Outeiro TF, Melki R, Kallunki P, Fog K, Li JY, Brundin P. α-Synuclein propagates from mouse brain to grafted dopaminergic neurons and seeds aggregation in cultured human cells. J Clin Invest. 2011 Feb 1 ;121(2):715-25. (recommandé par F1000)

- Espagne A, Erard M, Madiona K, Derrien V, Jonasson G, Lévy B, Pasquier H, Melki R, Mérola F. Cyan fluorescent protein carries a constitutive mutation that prevents its dimerization. Biochemistry. 2011 Feb 1 ;50(4):437-9.

- Noirot C, Habenstein B, Bousset L, Melki R, Meier BH, Endo Y, Penin F, Böckmann A. Wheat-germ cell-free production of prion proteins for solid-state NMR structural studies. N Biotechnol. 2011 Apr 30 ;28(3):232-8.


- Redeker V, Bonnefoy J, Le Caer JP, Pemberton S, Laprévote O, Melki R. A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry. FEBS J. 2010 Dec ;277(24):5112-23.

- Redeker V. Mass spectrometry analysis of C-terminal posttranslational modifications of tubulins. Methods Cell Biol. 2010 95:77-103.

- Caubet C, Bousset L, Clemmensen O, Sourigues Y, Bygum A, Chavanas S, Coudane F, Hsu CY, Betz RC, Melki R, Simon M, Serre G. A new amyloidosis caused by fibrillar aggregates of mutated corneodesmosin.
FASEB J. 2010 Sep ;24(9):3416-26.

- Brundin P, Melki R, Kopito R. Prion-like transmission of protein aggregates in neurodegenerative diseases. Nat Rev Mol Cell Biol. 2010 Apr ;11(4):301-7.

- Bousset L, Bonnefoy J, Sourigues Y, Wien F, Melki R. Structure and assembly properties of the N-terminal domain of the prion ure2p in isolation and in its natural context. PLoS One. Mar 22 ;5(3):e9760.

- Martineau CN, Melki R, Kabani M. Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ’Mat’ formation in the yeast Saccharomyces cerevisiae. FEBS Lett. 2010 584(6):1149-55.


- Loquet A, Bousset L, Gardiennet C, Sourigues Y, Wesmer C, Habenstein B, Schütz A, Meier BH, Melki R, Böckmann A. Prion fibrils of Ure2p assembled under physiological conditions contain highly ordered, natively folded modules. J Mol Biol. 2009 Nov 20 ;394(1):108-18.

- Sharma, D., Martineau, C. N., Le Dall, M. T., Reidy, M., Masison, D. C., and Kabani, M. Function of SSA subfamily of Hsp70 within and across species varies widely in complementing Saccharomyces cerevisiae cell growth and prion propagation. PLoS One 2009 Aug 14 ;4(8):e6644.

- Pieri L, Bucciantini M, Guasti P, Savistcheko J, Melki R, Stefani M. Synthetic lipid vesicles recruit native-like aggregates and affect the aggregation process of the prion Ure2p : insights on vesicle permeabilization and charge selectivity. Biophys J. 2009 Apr 22 ;96(8):3319-30.

- Ren, P. H., Lauckner, J. E., Kachirskaia, I., Heuser, J. E., Melki, R., and Kopito, R. R. Cytoplasmic penetration and persistent infection of mammalian cells by polyglutamine aggregates. Nat. Cell. Biol. 2009 11:219-225.

- Kabani, M., Structural and functional diversity among eukaryotic Hsp70 nucleotide exchange factors. Protein Peptide Lett. 2009 16(6):623-60.


- Kabani, M. and C.N. Martineau, Multiple Hsp70 isoforms in the eukaryotic cytosol : mere redundancy or functional specificity ? Curr Genomics, 2008. 9(5):338-348.

- Babour, A., M. Kabani, A. Boisrame, and J.M. Beckerich, Characterization of Ire1 in the yeast Yarrowia lipolytica reveals an important role for the Sls1 nucleotide exchange factor in unfolded protein response regulation. Curr Genet, 2008. 53(6) : p. 337-46.

- Bousset, L., J. Savistchenko, and R. Melki, Assembly of the Asparagine- and Glutamine-Rich Yeast Prions into Protein Fibrils Curr Alzheimer Res, 2008. 5(3) : p. 251-259.

- Savistchenko, J.,J. Krzewska, N. Fay, and R. Melki, Molecular chaperones and the assembly of the prion URE2p in vitro. J Biol Chem, 2008. 283 (23) : p. 15732-15739.

- Poh, S.L., F. El Khadali, C. Berrier, R. Lurz, R. Melki, and P. Tavares, Oligomerization of the SPP1 scaffolding protein. J Mol Biol, 2008. 378(3) : p. 551-64.


- Krzewska, J., M. Tanaka, S.G. Burston, and R. Melki, Biochemical and functional analysis of the assembly of full-length Sup35p and its prion-forming domain. J Biol Chem, 2007. 282(3) : p. 1679-86.

- Martineau, C.N., J.M. Beckerich, and M. Kabani, Flo11p-independent control of "mat" formation by hsp70 molecular chaperones and nucleotide exchange factors in yeast. Genetics, 2007. 177(3) : p. 1679-89.

- Redeker, V., F. Halgand, J.P. Le Caer, L. Bousset, O. Laprevote, and R. Melki, Hydrogen/deuterium exchange mass spectrometric analysis of conformational changes accompanying the assembly of the yeast prion Ure2p into protein fibrils. J Mol Biol, 2007. 369(4) : p. 1113-25.

- Tuite, M.F. and R. Melki, Protein Misfolding and Aggregation in Ageing and Disease : Molecular Processes and Therapeutic Perspectives. Prion, 2007. 2(2) : p. 116-120.


- Stirling, P.C., Cuellar, J., Alfaro, G.A., El Khadali, F., Beh, C.T., Valpuesta, J.M., Melki, R. and Leroux, M.R. (2006). "PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates" J Biol Chem, 281, 7012-7021.

- Sao-Jose, C., Lhuillier, S., Lurz, R., Melki, R., Lepault, J., Santos, M.A. and Tavares, P. (2006). "The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA" J Biol Chem, 281, 11464-11470.

- Ranson, N., Stromer, T., Bousset, L., Melki, R. and Serpell, L.C. (2006). "Insights into the architecture of the Ure2p yeast protein assemblies from helical twisted fibrils". Protein Sci., 15, 2481-2487

- Pucciarelli, S., Parker, S.K., Detrich, H.W., 3rd and Melki, R. (2006). "Characterization of the cytoplasmic chaperonin containing TCP-1 from the Antarctic fish Notothenia coriiceps". Extremophiles Dec ;10(6):537-49.

- Pieri, L., Bucciantini, M., Nosi, D., Formigli, L., Savistchenko, J., Melki, R. and Stefani, M. (2006). "The yeast prion Ure2p native-like assemblies are toxic to mammalian cells regardless of their aggregation state". J Biol Chem, 281, 15337-15344.

- Krzewska, J. and Melki, R. (2006). "Molecular chaperones and the assembly of the prion Sup35p, an in vitro study". Embo J, 25, 822-833. (recommandé par F1000)

- Fayard, B., Fay, N., David, G., Doucet, J. and Melki, R. (2006). "Packing of the prion Ure2p in protein fibrils probed by fluorescence X-ray near-edge structure spectroscopy at sulfur K-edge". J Mol Biol, 356, 843-849.

- Cecchini, M., Curcio, R., Pappalardo, M., Melki, R. and Caflisch, A. (2006). "A molecular dynamics approach to the structural characterization of amyloid aggregation". J Mol Biol, 357, 1306-1321.

- Jakubiec A, Tournier V, Drugeon G, Pflieger S, Camborde L, Vinh J, Hericourt F, Redeker V, Jupin I (2006). Phosphorylation of viral RNA-dependent RNA polymerase and its role in replication of a plus-strand RNA virus. J Biol Chem., 281, 21236-49.

- L. Bousset, N. Fay and R. Melki (2006) Template-induced protein misfolding underlying prion diseases. Topics in Current Genetics 16, 221-250


- Ghee, M., Melki, R., Michot, N. and Mallet, J. PA700, the regulatory complex of the 26S proteasome, interferes with alpha-synuclein assembly. Febs J. 2005 272 : 4023-4033.

- Fay, N., Redeker, V., Savistchenko, J., Dubois, S., Bousset, L. and Melki, R. Structure of the prion Ure2p in protein fibrils assembled in vitro. J Biol Chem. 2005 280 : 37149-37158.

- Bousset, L. and Melki, R. (2005). "[Prion proteins : folding and aggregation properties]". Med Sci (Paris) 21, 634-640.

- Redeker V, Levilliers N, Vinolo E, Rossier J, Jaillard D, Burnette D, Gaertig J, Bre MH (2005). Mutations of tubulin glycylation sites reveal cross-talk between the C termini of alpha- and beta-tubulin and affect the ciliary matrix in Tetrahymena. J Biol Chem, 280, 596-606.

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